PLA2G2A Antibody

• Catalog Number: orb1248077
• Category: Antibodies
• Description: PLA2G2A Antibody
• Target: PLA2G2A
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SYKFSNSGSRIT
• UniProt ID: P14555
• MW: Approx 19kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 19kDa band observed in Human Placenta lysates (calculated MW of 16.1kDa according to NP_000291.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PLA2G2A, phospholipase A2, group IIA (platelets, s
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_000291.1

orb1248077 (0.1 ug/ml) staining of Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248077 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248077 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.