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Catalog Number | orb1248077 |
---|---|
Category | Antibodies |
Description | PLA2G2A Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-SYKFSNSGSRIT |
Antibody Type | Primary Antibody |
Concentration | 500 ug/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 19kDa |
Target | PLA2G2A |
UniProt ID | P14555 |
NCBI | NP_000291.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PLA2G2A, phospholipase A2, group IIA (platelets, s Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 19kDa band observed in Human Placenta lysates (calculated MW of 16.1kDa according to NP_000291.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248077 (0.1 ug/ml) staining of Placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248077 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1248077 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.