PLA2G1B Antibody

• Catalog Number: orb1247092
• Category: Antibodies
• Description: PLA2G1B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: NKAHKNLDTKKYCQS
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PLA2G1B
• UniProt ID: P04054
• NCBI: NP_000919.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PLA2G1B, phospholipase A2, group IB (pancreas), MG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247092 (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PLA2G1B and probed with orb1247092 (mock transfection in first lane).