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PLA2G16 Antibody

SKU: orb1246537

Description

PLA2G16 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0kDa according to NP_009000.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: RAPIPEPKPGDLIE-C
TargetPLA2G16
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PLA2G16, phospholipase A2, group XVI, AdPLA, H-REV107-1, HRASLS3, HREV107, HREV107-3, MGC118754, HRAS-like suppressor 3, adipose-specific PLA2

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PLA2G16 Antibody

orb1246537 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PLA2G16 Antibody (orb1246537)

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0.1 mg
$ 920.00
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