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PKR (PRKR) Antibody (N-term)
Catalog Number: orb1939464
| Catalog Number | orb1939464 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PKR (PRKR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-42 amino acids from the N-terminal region of human PKR (PRKR). |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P19525 |
| MW | 62094 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
| Antibody Type | Primary Antibody |
| Clone Number | RB5281, RB5282 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Interferon-induced, double-stranded RNA-activated Read more... |
| Note | For research use only |
| NCBI | NP_002750.1, NP_001129123.1, NP_001129124.1 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-PRKR Pab is used in Western blot to detect PRKR in mouse liver tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2).
