PKP2 Rabbit Polyclonal Antibody

• Catalog Number: orb583224
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKP2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PKP2
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 93kDa
• Target: PKP2
• UniProt ID: A0AV37
• Protein Sequence: Synthetic peptide located within the following region: HPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPP
• NCBI: NP_001005242
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ARVD9
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Positive control (+): A549 cell lysate (A549), Negative control (-): U937 cell lysate (N31), Antibody concentration: 1 ug/ml.

Rabbit Anti-PKP2 Antibody, Catalog Number: orb583224, Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue, Observed Staining: Cytoplasmic, Primary Antibody Concentration: N/A, Other Working Concentrations: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-PKP2 Antibody, Positive Control: Lane1-4: 20 ug mouse keratinocyte lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit-HRP, Secondry Antibody dilution: 1:6000.

WB Suggested Anti-PKP2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: COLO205 cell lysate. PKP2 is supported by BioGPS gene expression data to be expressed in COLO205.