PKP2 Antibody

• Catalog Number: orb1249246
• Category: Antibodies
• Description: PKP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RGTAQYSSQKSVEER
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PKP2
• UniProt ID: Q99959
• NCBI: NP_001005242.2, NP_004563.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PKP2, plakophilin 2, ARVD9, MGC177501, plakophilin
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 100kDa band observed in Mouse Heart lysates (calculated MW of 88.1kDa according to Mouse NP_080439.1 and 87.8kDa according to Rat NP_001093969.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249246 (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249246 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.