PKM2 antibody

• Catalog Number: orb420361
• Category: Antibodies
• Description: PKM2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 5 µg/ml, WB: 1 ug/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-PKM2 is directed against human PKM2 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
• Conjugation: Unconjugated
• UniProt ID: P14618
• NCBI: NP_001193725.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.