PKM2 Antibody

• Catalog Number: orb251562
• Category: Antibodies
• Description: PKM2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2 (475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 57937 MW
• UniProt ID: P14618
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Pyruvate kinase PKM;2.7.1.40;Cytosolic thyroid hor
• Note: For research use only
• Application notes: WB: The detection limit for PKM2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PKM2 using anti-PKM2 antibody.Lane 1:human MCF-7 cell; 2:human MDA-B-453 cell; 3:human A549 cell; 4:human U87 cell; 5:rat brain tissue; 6:rat L6 cell; 7:mouse brain tissue; 8:mouse NIH/3T3 cell.

IF analysis of PKM2 using anti-PKM2 antibody. PKM2 was detected in an immunocytochemical section of A549 cells.

IHC analysis of PKM2 using anti-PKM2 antibody. PKM2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of PKM2 using anti-PKM2 antibody. PKM2 was detected in a paraffin-embedded section of human tonsil tissue.

IHC analysis of PKM2 using anti-PKM2 antibody. PKM2 was detected in a paraffin-embedded section of mouse skeletal muscle tissue.

IHC analysis of PKM2 using anti-PKM2 antibody. PKM2 was detected in a paraffin-embedded section of rat skeletal muscle tissue.