PKM antibody

• Catalog Number: orb527246
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKM
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3697
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:2000, IF: 1:200, IHC-P: 1:50- 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937
• Target: PKM
• UniProt ID: P14618
• NCBI: NP_001193728.1, NP_001193727.1, NP_001193726.1, NP_872270.1, NP_872271.1, NP_002645.3, NP_001193725.1
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody at 1:200 dilution.Secondary antibody was Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green).Nuclei counterstained with Hoechst 33342 (blue).

Western blot:All lanes:Anti-PKM2 Antibody (N491) at 1:1000 dilution.Lane 1:Hela;Lane 2:MCF-7.

Western blot analysis of hPKM2-N491 in A2058 cell line(lane 1) and mouse brain tissue(lane 2).PKM2 (arrow) was detected using the purified Pab.

Western blot:All lanes:Anti-hPKM2-N491 at 1:2000 dilution.Lane 1:Hela;Lane 2:A549;Lane 3:NIH/3T3;Lane 4:C2C12;Lane 5:PC-12.

Immunohistochemical analysis of human hepatocarcinoma using Pyruvate Kinase (PKM2) Antibody (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining.