PKM Antibody

• Catalog Number: orb1263426
• Category: Antibodies
• Description: PKM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: PKM
• UniProt ID: P14618
• NCBI: P14618
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of hPKM2-N491 in A2058 cell line (lane 1) and mouse brain tissue (lane 2) lysates (35 ug/lane)