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Catalog Number | orb1258900 |
---|---|
Category | Antibodies |
Description | PKM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM (NP_002645.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PKM |
UniProt ID | P14618 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kin Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PKM antibody (orb1258900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using PKM antibody (orb1258900) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PKM antibody (orb1258900) at dilution of 1:200 (40x lens).
ELISA, FC, IF, IHC, IP, WB | |
Human, Mouse, Rabbit, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, IP, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Bovine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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