PKCD antibody

• Catalog Number: orb154142
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKCD. Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calciumdependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteinerich C1 domains. Both novel and atypical PKCs are calciumindependent, but only novel PKCs are activated by PS, DAG, and phorbol esters (35). Members of these three PKC groups contain a pseudosubstrate or autoinhibitory domain that binds to substratebinding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxyterminal hydrophobic site Ser660 occurs in vivo (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKC (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKCrelated kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rhofamily GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human PKC delta(NP_006245.2).
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-100
• Conjugation: Unconjugated
• MW: 78kDa/85kDa
• UniProt ID: Q05655
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
• Alternative names: anti PRKCD antibody, anti MAY1 antibody, anti MGC4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines using PKCD antibody

Immunohistochemical staining of human liver cancer tissue using PKCD antibody (dilution of 1:100)

Immunohistochemical staining of human esophagus tissue using PKCD antibody (dilution of 1:100)