PKC eta Antibody

• Catalog Number: orb1263533
• Category: Antibodies
• Description: PKC eta Antibody
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PKC eta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the N-terminal region of human PKC eta.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: PRKCH
• UniProt ID: P24723
• NCBI: P24723
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein kinase C eta type, PKC-L, nPKC-eta, PRKCH,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of PRKCH using PKC eta Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCH gene (Lane 2).