PKC alpha/PRKCA Antibody

• Catalog Number: orb234364
• Category: Antibodies
• Description: PKC alpha/PRKCA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PKC alpha recombinant protein (Position: M153-L342). Human PKC alpha shares 97% and 99% amino acid (aa) sequence identity with mouse and rat PKC alpha, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 76750 MW
• UniProt ID: P17252
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein kinase C alpha type;PKC-A;PKC-alpha;2.7.11
• Note: For research use only
• Application notes: WB: The detection limit for PKC alpha is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PKC alpha using anti-PKC alpha antibody.Lane 1:human U87 cell; 2:human HEL cell; 3:human A549 cell; 4:human U20S cell; 5:rat brain tissue; 6:mouse brain tissue.

IHC analysis of PKC alpha using anti-PKC alpha antibody.PKC alpha was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of PKC alpha using anti-PKC alpha antibody.PKC alpha was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of PKC alpha using anti-PKC alpha antibody.PKC alpha was detected in paraffin-embedded section of human mammary cancer tissues.