PKA C alpha + beta Rabbit Polyclonal Antibody

• Catalog Number: orb6728
• Category: Antibodies
• Description: PKA C alpha + beta Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PKA beta (251-351/351aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40/50 kDa
• Target: PRKACA
• UniProt ID: P22694
• RRID: AB_10940847
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PKA C alpha+beta; PKA C alpha/beta; PKA C beta; PK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PKA C alpha+beta) Polyclonal Antibody, Unconjugated (orb6728) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Rat) Lysate at 40 ug, Primary: Anti-PKA C alpha+beta (orb6728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40/50 kD, Observed band size: 50 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Rat Heart tissue lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human Hela cell lysates, Lane 7: Human Huvec cell lysates, Primary: Anti-PKA C alpha + beta (orb6728) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40/50kD kDa, Observed band size: 50kD kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Testis (Rat) Lysate at 40 ug, Primary: Anti-PKA C alpha+beta (orb6728) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40/50 kD, Observed band size: 50 kD.