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PIWIL4 Antibody
SKU: orb1255367
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human PIWIL4 |
| Target | PIWIL4 |
| Molecular Weight | Observed: 110kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HIWI2, MIWI2, piwi-like protein 4, piwi-like 4, testis tissue sperm-binding protein Li 85P
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Western blot analysis of extracts of various cell lines, using PIWIL4 antibody (orb1255367) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using PIWIL4 antibody (orb1255367) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PIWIL4 antibody (orb1255367) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PIWIL4 antibody (orb1255367). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
PIWIL4
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
PIWIL4 Antibody (orb1255367)
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