Piwil2 Rabbit Polyclonal Antibody

• Catalog Number: orb6086
• Category: Antibodies
• Description: Piwil2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Guinea pig, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Piwil2/Mili (885-973/973aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=0.2μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 107 kDa
• Target: PIWIL2
• UniProt ID: Q8TC59
• RRID: AB_10917183
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PIWL2_HUMAN; Piwi-like protein 2; EC:3.1.26.-; HIL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Piwil2 antibody (orb6086), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Testis (Mouse) Lysate at 40 ug, Testis (Rat) Lysate at 40 ug, HepG2 (Human) Cell Lysate at 30 ug, Lovo (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Piwil2 (orb6086) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 107 kD, Observed band size: 110 kD.