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Catalog Number | orb1239221 |
---|---|
Category | Antibodies |
Description | PIWI-L1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Gallus |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.The immunogen is located within amino acids 60 - 110 of PIWI-L1. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 85, 90 kDa Observed: 95 kDa |
Target | PIWIL1 |
UniProt ID | Q96J94 |
NCBI | AAK69348 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Read more... |
Note | For research use only |
Application notes | PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 µg/mL.
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 µg/mL.