PIWIL1 Antibody

SKU: orb1239221

Description

PIWIL1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus
• Application Notes:
  PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.The immunogen is located within amino acids 60 - 110 of PIWI-L1.
• Target: PIWIL1
• Molecular Weight: Predicted: 85, 90 kDa Observed: 95 kDa
• Purification: PIWI-L1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1

Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 µg/mL.

Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

PIWIL1

UniProt

Q96J94

RefSeq:AAK69348