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| Catalog Number | orb1239221 |
|---|---|
| Category | Antibodies |
| Description | PIWI-L1 Antibody |
| Target | PIWIL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Gallus |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | PIWI-L1 Antibody is affinity chromatography purified via peptide column. |
| Immunogen | PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.The immunogen is located within amino acids 60 - 110 of PIWI-L1. |
| UniProt ID | Q96J94 |
| MW | Predicted: 85, 90 kDa Observed: 95 kDa |
| Tested applications | ELISA, IF, IHC-P, WB |
| Application notes | PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Read more... |
| Note | For research use only |
| NCBI | AAK69348 |

Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 µg/mL.

Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 µg/mL.
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