PITPNM3 Antibody

• Catalog Number: orb1247094
• Category: Antibodies
• Description: PITPNM3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Canine, Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SWARGPPKFESVP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1ug/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PITPNM3
• UniProt ID: Q9BZ71
• NCBI: NP_001159438.1, NP_112497.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PITPNM3, NIR1, PITPNM family member 3, PYK2 N-term
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1ug/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247094 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.