Pioglitazone

SKU: orb1310828

Description

Pioglitazone is a potent and selective PPARγ agonist with EC50 values of 0.93 μM (human) and 0.99 μM (mouse). This orally active small molecule is widely used in diabetes research for in vitro mechanistic studies and in vivo models of insulin resistance and glucose homeostasis.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 111025-46-8
• MW: 356.44
• Purity: 99.57% (May vary between batches)
• Formula: C₁₉H₂₀N₂O₃S
• SMILES: C(C1C(=O)NC(=O)S1)C2=CC=C(OCCC3=CC=C(CC)C=N3)C=C2
• Target: Ferroptosis,PPAR
• Solubility: 10% DMSO+90% Corn Oil:2 mg/mL (5.61 mM);DMSO:28.8 mg/mL (80.8 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PPARδ (human):43 μM (EC50)|PPARγ (mouse):0.99 μM (EC50)|PPARα (mouse):100 μM (EC50)|PPARα (human):100 μM (EC50)|PPARγ (human):0.93 μM (EC50)
• In Vivo:
  METHODS: To investigate the effect of Pioglitazone on insulin resistance, Pioglitazone (10 and 30 mg/kg) was orally administered to ob/ob and adipo-/-ob/ob mice once daily for 14 days. RESULTS: Pioglitazone improves insulin resistance and diabetes, which may be lipocalin-dependent in the liver but not in skeletal muscle. METHODS: To investigate the effect of Pioglitazone on cardiac remodeling, diabetic nephropathy rats were treated with oral administration of Pioglitazone (10 mg/kg) once daily for 4 weeks. RESULTS: Pioglitazone significantly reduced body weight (BW), cardiac hypertrophy, elevated blood glucose levels, and related dyslipidemia.
• In Vitro:
  METHODS: HT-1080, MDA-MB-231, and PC-3 cells were treated with Pioglitazone for 3 days, and target cell toxicity was measured using MTT assay. RESULTS: Pioglitazone did not affect cell growth at 100 μM.

Storage & Handling

• Storage: store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pioglitazone, PPARγ, PPAR, Peroxisome proliferator-activated receptors, U72107, U-72107, U 72107, inhibit, ligand-binding, Ferroptosis, Inhibitor, diabete, domain, blood glucose

Compound Identifiers

PubChem CID

4829