PIK3R1 monoclonal antibody (M01), clone 3A10

• Catalog Number: orb2292401
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant PIK3R1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3A10
• Tested applications: ELISA, IP, PLA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1 Kappa
• Immunogen: PIK3R1 (AAH30815, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
• NCBI: AAH30815
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in Raw 264.7.

Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1 ng/ml as a capture antibody.

Immunoprecipitation of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.

PIK3R1 monoclonal antibody (M01), clone 3A10 Western Blot analysis of PIK3R1 expression in HeLa.

PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in NIH/3T3.

PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in PC-12.

Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10. Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (75.68 KDa).