PIK3CA antibody

• Catalog Number: orb19357
• Category: Antibodies
• Description: Goat polyclonal antibody to PIK3CA
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 1 μg/ml)., IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 124
• Target: PIK3CA / P110alpha
• Entrez: 5290
• Protein Sequence: HQWLKDKNKGEIYD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PIK3CA antibody, anti p110-alpha antibody, an
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml.Immunohistochemistry data: see also publciation Abubaker J et al. Mol Cancer. 2009 Jul 28;8:51. (PMID: 19638206).WB: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of paraffin embedded Human Brain (Cerebellar Cortex) using PIK3CA antibody

Immunohistochemical staining of Human Heart using PIK3CA antibody