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Catalog Number | orb1260896 |
---|---|
Category | Antibodies |
Description | PIK3CA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human PIK3CA |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 110kDa |
Target | PIK3CA |
UniProt ID | P42336 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Phosphatidylinositol 4,5-bisphosphate 3-kinase cat Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of paraffin-embedded human stomach using PIK3CA antibody (orb1260896) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse kidney, using PIK3CA Antibody (orb1260896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using PIK3CA antibody (orb1260896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PIK3CA antibody (orb1260896) at dilution of 1:100 (40x lens).
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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