PIK3CA Antibody: Biotin

• Catalog Number: orb414118
• Category: Antibodies
• Description: Rabbit polyclonal antibody against PIK3CA conjugated to Biotin
• Target: PIK3CA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide of Human PIK3CA (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• UniProt ID: P42336
• MW: 55.7 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000)
• Application notes: A 1:1000 dilution of SPC-741 was sufficient for detection of PIK3CA in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: PI 3 Kinase catalytic subunit alpha antibody, Phos
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only
• Entrez: 5290
• NCBI: NP_006209.2

Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.