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PIK3 gamma Rabbit Polyclonal Antibody

Catalog Number: orb158154

DispatchUsually dispatched within 5-10 working days
$ 240.00
Catalog Numberorb158154
CategoryAntibodies
DescriptionPIK3 gamma Rabbit Polyclonal Antibody
TargetPIK3CG
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rabbit, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Purificationaffinity purified by Protein A
ImmunogenKLH conjugated synthetic peptide derived from human PIK3 gamma (521-620/1102aa)
UniProt IDP48736
MW121 kDa
Tested applicationsFC
Dilution rangeFlow-Cyt=1ug/test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPK3CG_HUMAN; PI 3 Kinase catalytic subunit gamma;
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NoteFor research use only
PIK3 gamma Rabbit Polyclonal Antibody

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-PIK3 gamma/FITC Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.