PIGR Rabbit Polyclonal Antibody

• Catalog Number: orb100319
• Category: Antibodies
• Description: PIGR Rabbit Polyclonal Antibody
• Target: PIGR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PIGR (31-130/764aa)
• UniProt ID: P01833
• MW: 81 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Hepatocellular carcinoma associated protein TB6; P
• Note: For research use only

References

Bhaskara, Venugopal et al. Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells J Biotechnol, 331, (2021)

PubMed: 33689865

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (orb100319) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minu.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-PIGR (orb100319) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 131 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-PIGR (orb100319) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 131 kD.