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PIEZO2 Rabbit Polyclonal Antibody

Catalog Number: orb325498

DispatchUsually dispatched within 1 - 2 weeks
$ 600.00
Catalog Numberorb325498
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PIEZO2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsWB
Predicted ReactivityBovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
ReactivityHuman
ImmunogenThe immunogen is a synthetic peptide directed towards the N-terminal region of Human PIEZO2
Concentration0.5 mg/ml
Form/AppearanceLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ConjugationUnconjugated
MW77 kDa
TargetPIEZO2
UniProt IDQ9H5I5
Protein SequenceSynthetic peptide located within the following region: VFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRK
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Alternative namesDA3, DA5, MWKS, DAIPT, FAM38B, HsT748, HsT771, FAM
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NoteFor research use only
Expiration Date12 months from date of receipt.
PIEZO2 Rabbit Polyclonal Antibody

Sample Type: 293T Whole Cell lysates, Antibody Dilution: 1.0 ug/mL.

PIEZO2 Rabbit Polyclonal Antibody

Positive control (+): Human Ovary (OV), Negative control (-): Human pancreas (PA), Antibody concentration: 1 ug/mL.

PIEZO2 Rabbit Polyclonal Antibody

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

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