PI31 Antibody

• Catalog Number: orb1247105
• Category: Antibodies
• Description: PI31 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DHLPPPGYDDMYL
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PSMF1
• UniProt ID: Q92530
• NCBI: NP_006805.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PSMF1, PI31, proteasome (prosome, macropain) inhib
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247105 staining (1 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing PSMF1 and probed with orb1247105 (mock transfection in first lane).