PI31/PSMF1 (Isoform 1) Antibody

SKU: orb18428

Description

Goat polyclonal to PI31

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1μg/ml, IF/ICC: 10μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PI31 / PSMF1 (Isoform 1)
• Molecular Weight: 29.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PSMF1 antibody, anti PI31 antibody, anti proteasome (prosome, macropain) inhibitor subunit 1 (PI31) antibody, anti RP4-545L17.1 antibody, anti proteasome inhibitor hP131 subunit antibody, anti proteasome inhibitor subunit 1 antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using PI31 antibody.

Western blot analysis of KNRK (A), NIH3T3 (B and (0.3ug/ml) Neuro2a (C) cell lysate using PI31 antibody.

Western blot analysis of HEK293 (A), K562 (B) and (1ug/ml) LNCaP (C) cell lysate using PI31 antibody.

Quick Database Links

Gene Symbol

PI31 / PSMF1 (Isoform 1)

NCBI Gene ID

9491