PI3 Kinase p110 delta Rabbit Polyclonal Antibody

SKU: orb185886

Description

PI3 Kinase p110 delta Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PI3 Kinase p110 delta (51-150/1044aa)
• Target: PIK3CD
• Molecular Weight: 115 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APDS; IMD14; IMD14A; IMD14B; P110DELTA; PI3K; ROCHIS; p110D; 2410099E07Rik; 2610208K16Rik; PK3CD_HUMAN; PIK3CD; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta (PtdIns-3-kinase subunit p110-delta | p110delta); 2.7.1.137; 2.7.1.153; PK3CD_MOUSE;

Sample: Lane 1: Human HT-1080 cell lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-PI3 Kinase p110 delta (orb185886) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kDa, Observed band size: 120 kDa.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-PI3 Kinase p110 delta (orb185886) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated (orb185886) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PIK3CD

UniProt

O00329