PI-9 rabbit pAb

• Catalog Number: orb766077
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PI-9
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human SERPINB9. AA range:241-290
• MW: 40
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-SERPINB9 antibody, anti-PI9 antibody, anti-Se
• Note: For research use only

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of various cells using PI-9 Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO, HUVEC, and Jurkat cells, using SERPINB9 Antibody. The lane on the right is blocked with the synthesized peptide.