Phosphos-PCNA (Tyr211) Rabbit Polyclonal Antibody

SKU: orb5867

Description

Phosphos-PCNA (Tyr211) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1μg /test
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus, Mouse, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PCNA around the phosphorylation site of Tyr211 LR(p-Y)LN
• Target: PCNA
• Molecular Weight: 29 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PCNA(phospho Y211); p-PCNA(phospho Y211); PCNA(Tyr211); PCNA; Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen; PCNA_HUMAN.

Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-PCNA antibody (orb5867), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phosphos-PCNA (Tyr211)) polyclonal Antibody, Unconjugated (orb5867) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004