Phosphor-PPP3CA (Ser469) Rabbit Polyclonal Antibody

• Catalog Number: orb185888
• Category: Antibodies
• Description: Phosphor-PPP3CA (Ser469) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Gallus, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PPP3CA around the phosphorylation site of Ser469 (421-521/521aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:25, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: PPP3CA
• UniProt ID: Q08209
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PPP3CA(phosphor S469); PPP3CA(phosphor Ser469); Ca
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phosphor-PPP3CA (Ser469)) polyclonal Antibody, Unconjugated (orb185888) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPP3CA (Ser469)) Polyclonal Antibody, Unconjugated (orb185888) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.