PDE4B Antibody

SKU: orb1247106

Description

PDE4B Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 µg/mL.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DIDIATEDKSPVDT
• Target: PDE4B
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDE4B, phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila), RP5-876C12.1, DKFZp686F2182, DPDE4, MGC126529, PDEIVB , OTTHUMP00000046896, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, dunce-like phosphodiesterase E4, phosphodiesterase 4B, cAMP-specific, phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E4)

orb1247106 (0.5 ug/ml) staining of Mouse Brain Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human PDE4B and probed with orb1247106 (mock transfection in first lane).

orb1247106 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PDE4B

UniProt

Q07343

RefSeq (Protein):NP_002591.2, NP_001032416.1, NP_001032418.1, NP_001032417.1