Phospho-ZIP Kinase (Thr265) Rabbit Polyclonal Antibody

• Catalog Number: orb318041
• Category: Antibodies
• Description: Phospho-ZIP Kinase (Thr265) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265 RM(p-T)IA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: DAPK3
• UniProt ID: O43293
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: ZIP Kinase(phospho T265); p-ZIP Kinase(phospho T26
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-ZIP Kinase (Thr265)) Polyclonal Antibody, Unconjugated (orb318041) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-phospho-ZIP Kinase (Thr265) (orb318041) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-phospho-ZIP Kinase (Thr265) (orb318041) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.