Phospho-ZAP70 (Tyr493) Rabbit Polyclonal Antibody (FITC)

SKU: orb9989

Description

Phospho-ZAP70 (Tyr493) Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting ZAP70. This antibody is suitable for ICC. It exhibits reactivity with Human samples.

Research Area

CD Markers, Cancer Research, Cell Biology, SARS /COVID-19, Tumor Biology, Tyrosine Kinases

Images & Validation

• Tested Applications: ICC
• Dilution range: ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr493 SY(p-Y)TA
• Target: ZAP70
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Zap-70(phospho-Tyr493); ZAP70(phospho Y493); p-ZAP70(Y292); p-Zap-70(Tyr292); p-Zap-70(Y292); ZAP-70; ZAP 70; ZAP70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein.

Paraformaldehyde-fixed, paraffin embedded (Rat lymph nodes), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Phospho-ZAP70 (Tyr493) Antibody, conjugated (orb9989) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Phospho-ZAP70 (Tyr493) Antibody, conjugated (orb9989) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

ZAP70