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Item 1 of 2
Phospho-ZAP70 (Tyr493) Rabbit Polyclonal Antibody (FITC)
Catalog Number: orb9989
| Catalog Number | orb9989 |
|---|---|
| Category | Antibodies |
| Description | Phospho-ZAP70 (Tyr493) Rabbit Polyclonal Antibody (FITC) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ICC |
| Predicted Reactivity | Bovine, Equine, Mouse, Porcine, Rabbit, Rat |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr493 SY(p-Y)TA |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | ICC/IF=1:100-500 |
| Conjugation | FITC |
| Target | ZAP70 |
| RRID | AB_10918213 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Zap-70(Phospho-Tyr493); ZAP70 (phospho Y493); p-ZA Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Rat lymph nodes), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Phospho-ZAP70 (Tyr493) Antibody, conjugated (orb9989) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Phospho-ZAP70 (Tyr493) Antibody, conjugated (orb9989) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
