Phospho-ZAP70 (Tyr292) Rabbit Polyclonal Antibody

SKU: orb158734

Description

Phospho-ZAP70 (Tyr292) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr292 DG(p-Y)TP
• Target: ZAP70
• Molecular Weight: 68 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ZAP70(phospho Y292); ZAP70(phospho Tyr292); p-Zap-70(Tyr292); p-Zap-70(Y292); p-ZAP70(Y292); p-ZAP70(Tyr292); ZAP-70; ZAP 70; ZAP70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein.

Molt-4 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZAP-70 Antibody (orb158734) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Sample: Lane 1: Human Jurkat-PV cell lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-phospho-ZAP70 (Tyr292) (orb158734) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 65 kDa.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-p-ZAP70 (Tyr292) (orb158734) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68kD, Observed band size: 68kD.

Quick Database Links

Gene Symbol

ZAP70

UniProt

P43403