Phospho-ZAP70 (Tyr292) Rabbit Polyclonal Antibody

• Catalog Number: orb158734
• Category: Antibodies
• Description: Phospho-ZAP70 (Tyr292) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyr292 DG(p-Y)TP
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: ZAP70
• UniProt ID: P43403
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ZAP70(phospho Y292); ZAP70(phospho Tyr292); p-Zap-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Molt-4 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZAP-70 Antibody (orb158734) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Sample: Lane 1: Human Jurkat-PV cell lysates, Lane 2: Human Jurkat cell lysates, Primary: Anti-phospho-ZAP70 (Tyr292) (orb158734) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 65 kDa.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-p-ZAP70 (Tyr292) (orb158734) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68kD, Observed band size: 68kD.