Phospho-VEGFR2 (Tyr1175) Rabbit Polyclonal Antibody

• Catalog Number: orb14809
• Category: Antibodies
• Description: Phospho-VEGFR2 (Tyr1175) Rabbit Polyclonal Antibody
• Target: KDR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human VEGFR2 around the phosphorylation site of Tyr1175 KD(p-Y)IV
• UniProt ID: P35968
• RRID: AB_10752889
• MW: 147 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VEGFR2(phospho-Tyr1175); VEGFR2(phospho-Y1175); p-
• Note: For research use only

References

Pal, Sumanta K. et al. RNA-seq reveals aurora kinase-driven mTOR pathway activation in patients with sarcomatoid metastatic renal cell carcinoma Mol Cancer Res, 13, 130-137 (2015)

PubMed: 25183163

Blank control: Huvec. Primary Antibody (green line): Rabbit Anti-phospho-VEGFR2 (Tyr1175) antibody (orb14809), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Adrenal gland (Rat) Lysate at 40 ug, Primary: Anti-phospho-VEGFR2 (Tyr1175) (orb14809) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 147 kD, Observed band size: 220 kD.