Phospho-TrkA (Tyr496) +TrkB (Tyr516) Rabbit Polyclonal Antibody

SKU: orb7119

Description

Phospho-TrkA (Tyr496) +TrkB (Tyr516) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TrkA around the phosphorylation site of Tyr496 PQ(p-Y)F
• Target: NTRK1
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TrkA(phospho-Tyr496); TrkA(phospho Tyr496); TrkA(phospho-Y496); TrkA(phospho Y496); p-TrkA(Tyr496); TrkB(phospho-Tyr516); TrkB(phospho Tyr516); TrkB(phospho-Y516); p-TrkB(Tyr516); CIPA; CIPA; DKFZp781I14186; gp140trk; High affinity nerve growth factor receptor; High affinity nerve growth factor receptor precursor; High affinity nerve growth factor receptor precursor; MTC; MTC; neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase receptor type 1; Ntrk 1; Ntrk1; Ntrk1; NTRK1_HUMAN; Oncogene TRK; Oncogene TRK; p140 TrkA; p140-TrkA; TRK 1; Trk A; TRK; TRK; Trk-A; TRK1; TRK1 transforming tyrosine kinase protein; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor A; Tyrosine kinase receptor; Tyrosine Receptor Kinase A.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Phospho-TrkA (Tyr496)+TrkB (Tyr516) antibody (orb7119), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-TrkA (Tyr496) +TrkB (Tyr516)) Polyclonal Antibody, Unconjugated (orb7119) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-TrkA (Tyr496) +TrkB (Tyr516) (orb7119) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Quick Database Links

Gene Symbol

NTRK1

UniProt

P04629