Phospho-TCF3 (Ser39) Rabbit Polyclonal Antibody

SKU: orb100515

Description

Phospho-TCF3 (Ser39) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human TCF3 around the phosphorylation site of Ser39 PA(p-S)LA
• Target: TCF3
• Molecular Weight: 67 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCF3 (p-S39); p-TCF3; phospho-TCF3; AGM8; AGM8A; AGM8B; E2A; E47; ITF1; TCF-3; VDIR; bHLHb21; p75; A1; ALF2; E12; E12/E47; KA1; ME2; Pan1; Pan2; Tcfe2a; TFE2_HUMAN; TCF3; Class B basic helix-loop-helix protein 21 (bHLHb21); Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3 (TCF-3); Transcription factor ITF-1; TFE2_MOUSE; Transcription factor A1; TFE2_RAT; Pancreas specific transcription factor 1c; Transcription regulator Pan; Pan; Ptf1c;

Sample: Embryo (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, Primary: Anti-phospho-TCF3 (Ser39) (orb100515) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 67 kD, Observed band size: 68 kD.

Tissue/cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-phospho-TCF3/E2A/E47 (Ser39) Polyclonal Antibody, Unconjugated (orb100515) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TCF3

UniProt

P15923