Phospho-Tau (Thr522) Rabbit Polyclonal Antibody

• Catalog Number: orb158570
• Category: Antibodies
• Description: Phospho-Tau (Thr522) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser522 PG(p-T)PG
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52/79 kDa
• Target: MAPT
• UniProt ID: P10636
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tau(phospho-Thr522); MAPT(phospho T522); p-Tau pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tau (Thr522)) Polyclonal Antibody, Unconjugated (orb158570) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: BV-2 (Mouse) Cell Lysate at 30 ug Primary: Anti-phospho-Tau (Thr522) (orb158570) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52/79 kD Observed band size: 52/129 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Thr522) (orb158570) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 50 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Thr522) (orb158570) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52/79 kD, Observed band size: 52 kD.