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Phospho-Tau (Ser721) Rabbit Polyclonal Antibody

Catalog Number: orb7062

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb7062
CategoryAntibodies
DescriptionPhospho-Tau (Ser721) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser721 DT(p-S)PR
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW52/79 kDa
TargetMAPT
UniProt IDP10636
RRIDAB_10921141
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMAPT(phospho S721); MAPT; Microtuble-associted pro
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-Tau (Ser721) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tau (Ser721)) Polyclonal Antibody, Unconjugated (orb7062) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Tau (Ser721) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tau (Ser721)) Polyclonal Antibody, Unconjugated (orb7062) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Tau (Ser721) Rabbit Polyclonal Antibody

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: Cerebellum (Mouse) Lysate at 40 ug, Lane 4: Cerebellum (Rat) Lysate at 40 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau (Ser721) (orb7062) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 50 kD.

Phospho-Tau (Ser721) Rabbit Polyclonal Antibody

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Tau (phospho-Tau (Ser721)) polyclonal Antibody, Unconjugated (orb7062) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.