Phospho-Tau (Ser416) Rabbit Polyclonal Antibody

• Catalog Number: orb7069
• Category: Antibodies
• Description: Phospho-Tau (Ser416) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser416 TG(p-S)ID
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52/79 kDa
• Target: MAPT
• UniProt ID: P10636
• RRID: AB_10926069
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tau(phospho S416); p-Tau(phospho S416); MAPT(phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): MCF 7 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Tau (Ser416) antibody (orb7069), dilution: 1 µg/10^5 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52/79 kD, Observed band size: 52 kD.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 55'62kD.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser416) (orb7069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 55'62kD.