Phospho-Tau (Ser404) Rabbit Polyclonal Antibody

• Catalog Number: orb7060
• Category: Antibodies
• Description: Phospho-Tau (Ser404) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser404 IG(p-S)TE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52/79 kDa
• Target: MAPT
• UniProt ID: P10636
• RRID: AB_10930991
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tau(phospho S404); p-Tau(phospho S404); Tau(phosph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: SH-SY5Y (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau (orb7060) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52'79 kD, Observed band size: 52 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: Cerebellum (Mouse) Lysate at 40 ug, Lane 4: Cerebellum (Rat) Lysate at 40 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau (Ser404) (orb7060) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 50 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-Tau protein (Ser404) Polyclonal Antibody, Unconjugated (orb7060) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.