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Phospho-Tau (Ser396) Rabbit Polyclonal Antibody

Catalog Number: orb7057

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb7057
CategoryAntibodies
DescriptionPhospho-Tau (Ser396) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser396 KT(p-S)T
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW46/79 kDa
TargetMAPT
UniProt IDP10636
RRIDAB_10933495
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesTau(phospho S396); p-Tau(phospho S396); Tau(phosph
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-Tau (Ser396) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Tau (Ser396)) Polyclonal Antibody, Unconjugated (orb7057) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Tau (Ser396) Rabbit Polyclonal Antibody

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Ser396) (orb7057) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52/79 kD, Observed band size: 52 kD.

Phospho-Tau (Ser396) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-Tau protein (Ser396) Polyclonal Antibody, Unconjugated (orb7057) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.