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Catalog Number | orb7051 |
---|---|
Category | Antibodies |
Description | Phospho-TAK1 (Ser192) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC-Fr, IHC-P |
Predicted Reactivity | Bovine, Equine, Gallus, Mouse, Porcine, Rabbit |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human TAK1 around the phosphorylation site of Ser192 KG(p-S)AA |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 67 kDa |
Target | MAP3K7 |
UniProt ID | O43318 |
RRID | AB_10941508 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | MAP3K7(phospho S192); TAK1(phospho Ser192); MAP3K7 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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Pham, Thu Thao et al. MicroRNA gga-miR-10a-mediated transcriptional regulation of the immune genes in necrotic enteritis afflicted chickens Dev Comp Immunol, 102, 103472 (2020)
Applications
Reactivity
Hong, Yeojin et al. Exosomes of lipopolysaccharide-stimulated chicken macrophages modulate immune response through the MyD88/NF-κB signaling pathway Dev Comp Immunol, 115, 103908 (2021)
Applications
Reactivity
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-TAK1 (Ser192)) polyclonal Antibody, Unconjugated (orb7051) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-TAK1 (Ser192) polyclonal Antibody, Unconjugated (orb7051) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
ICC, IF | |
Bovine, Equine, Gallus, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
PE/Cy7 |
IHC-Fr, IHC-P | |
Bovine, Equine, Gallus, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
AP |
ICC, IF | |
Bovine, Equine, Gallus, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
PE/Cy5.5 |
ICC, IF | |
Bovine, Equine, Gallus, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Cy3 |
ICC, IF, IHC-Fr, IHC-P | |
Bovine, Equine, Gallus, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Biotin |