Phospho-TAK1 (Ser192) Rabbit Polyclonal Antibody

• Catalog Number: orb7051
• Category: Antibodies
• Description: Phospho-TAK1 (Ser192) Rabbit Polyclonal Antibody
• Target: MAP3K7
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TAK1 around the phosphorylation site of Ser192 KG(p-S)AA
• UniProt ID: O43318
• RRID: AB_10941508
• MW: 67 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAP3K7(phospho S192); TAK1(phospho Ser192); MAP3K7
• Research Area: Cancer Research, Cell Biology, Inflammation, Infla
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Pham, Thu Thao et al. MicroRNA gga-miR-10a-mediated transcriptional regulation of the immune genes in necrotic enteritis afflicted chickens Dev Comp Immunol, 102, 103472 (2020)

PubMed: 31437523

Applications

WB

Reactivity

Gallus

Hong, Yeojin et al. Exosomes of lipopolysaccharide-stimulated chicken macrophages modulate immune response through the MyD88/NF-κB signaling pathway Dev Comp Immunol, 115, 103908 (2021)

PubMed: 33115603

Applications

IHC, WB

Reactivity

Gallus

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-TAK1 (Ser192)) polyclonal Antibody, Unconjugated (orb7051) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-TAK1 (Ser192) polyclonal Antibody, Unconjugated (orb7051) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.