Phospho-STMN1 (Ser25) Rabbit Polyclonal Antibody

SKU: orb6290

Description

Phospho-STMN1 (Ser25) Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser25 IL(p-S)PR
• Target: STMN1
• Molecular Weight: 17 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

STMN1 (p-S25); p-STMN1; phospho-STMN1; C1orf215; LAP18; Lag; OP18; PP17; PP19; PR22; SMN; 19k; P18; P19; Pig; Pp18; prosolin; STMN1_HUMAN; STMN1; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18 (Op18); Phosphoprotein p19 (pp19); Protein Pr22; STMN1_MOUSE; Leukemia-associated gene protein; STMN1_RAT; Pr22 protein;

Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (hospho-STMN1 (Ser25)) Polyclonal Antibody, Unconjugated (orb6290) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Stathmin 1 (Ser25) (orb6290) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 19 kD.

Quick Database Links

Gene Symbol

STMN1

UniProt

P16949