Phospho-STAT5a(S726) Antibody

• Catalog Number: orb1931217
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5945
• Tested applications: DOT, IHC-P, WB
• Predicted Reactivity: Porcine, Sheep
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, DB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 90647 Da
• Target: This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S726 of human STAT5a.
• UniProt ID: P42229
• NCBI: NP_003143.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Signal transducer and activator of transcription 5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of anti-Phospho-hSTAT5a-pS726 Phospho-specific Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-Phospho-STAT5a-S726 Antibody in CEM cell line lysate (35 ug/lane). Phospho-STAT5a (arrow) was detected using the purified Pab.