Phospho-Src (Tyr215) Rabbit Polyclonal Antibody

SKU: orb7006

Description

Phospho-Src (Tyr215) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC
• Dilution range: ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Canine, Gallus, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse Src around the phosphorylation site of Tyr215 GF(p-Y)IT
• Target: SRC
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SRC(phospho Tyr215); Src(phospho Y215); p-Src(phospho Y215); ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_HUMAN; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma(Schmidt Ruppin A2) viral oncogene homolog; V src sarcoma(Schmidt Ruppin A 2) viral oncogene homolog(avian); v src sarcoma(Schmidt Ruppin A 2) viral oncogene homolog avian; vsrc avian sarcoma(Schmidt Ruppin A2) viral oncogene homolog.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Src (Tyr215)) polyclonal Antibody, Unconjugated (orb7006) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

SRC

UniProt

P05480