Phospho-SMAD5 (Ser463 + Ser465) Rabbit Polyclonal Antibody

SKU: orb313113

Description

Phospho-SMAD5 (Ser463 + Ser465) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Signaling, Cytoplasmic, Intracellular Signaling Proteins, Liver Development

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465 IS(p-S)V(p-S)
• Target: SMAD5
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMAD5(phospho S463+S465); p-SMAD5(phospho S463+S465); DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; Smad5; Smad5; SMAD5_HUMAN.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-p-SMAD5 (Ser463+Ser465) (orb313113) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Quick Database Links

Gene Symbol

SMAD5

UniProt

Q99717